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rabbit α suv3  (Fortis Life Sciences)
93
Fortis Life Sciences rabbit α suv3
(A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, <t>SUV3,</t> and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.
Rabbit α Suv3, supplied by Fortis Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+%CE%B1+suv3/SUV3+Antibody/bio_rxiv__2025__08__04__668575-291-24-26
Average 93 stars, based on 1 article reviews
rabbit α suv3 - by Bioz Stars, 2026-11
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(A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, SUV3, and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.

Journal: bioRxiv

Article Title: RNA triggers chronic stress during neuronal aging

doi: 10.1101/2025.08.04.668575

Figure Lengend Snippet: (A) Screen captures from the Agilent TapeStation Analysis Software v4.1.1 of RNA ScreenTapes loaded with samples from throughout the dsRNA purification protocol in HEK293T cells. Exogenous (exo.) dsRNA of a known size (∼150 bp) was tracked throughout the purification. (B) Volcano plot of the log 2 (Fold Change) of all MT-RNA transcripts identified by dsRNA-Seq in the input and immunoprecipitation (IP) samples of the Tdiff.2 transdifferentiated neuron line. (C) Same as (B) but for Tdiff.5 transdifferentiated neurons. (D) Same as (B) but for iPSC-diff.1 neurons. (E) Volcano plot of RNA-seq in transdifferentiated neurons (Tdiff.1/2/4/5) versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). Orange data points denote nuclear-encoded mitochondrial genes whereas yellow data points denote MT-RNA transcripts. The MT-ND6, SUV3, and PNPT1 transcripts are indicated on the plot. (F) Boxplot of the Log 2 (Fold Change) of all transcripts (gray), nuclear-encoded mitochondrial transcripts (orange), and MT-RNA transcripts (yellow) in transdifferentiated neurons versus iPSC-derived neurons (n=3 replicates per line; N=4 lines per cohort). (G) Visualization of representative mitochondrial sequencing reads detected by dsRNA-Seq and PKR CLIP in the Tdiff.1 transdifferentiated neuron line. The shaded region denotes the reverse-transcribed MT-ND6 gene. The range of the y-axis values is denoted on the right side of the genomic track and was fixed for each pair of input and IP samples.

Article Snippet: The following antibodies were used for eCLIP: 1 μg Rabbit α-PKR (Cell Signaling Technology #12297S); 10 μg Rabbit α-PNPT1 (Bethyl #A303-917A); and 2 μg Rabbit α-SUV3 (Bethyl #A303-056A).

Techniques: Software, Purification, Immunoprecipitation, RNA Sequencing, Derivative Assay, Sequencing, Reverse Transcription

(A) Additional airyscan immunofluorescence-FISH images of the mitochondrial marker TOM70 (magenta) and MT-ND6 transcript (green) in Tdiff.1 neurons. Green arrowheads denote cytoplasmic MT-ND6 puncta; the white arrows denote the intensity profiles in the adjacent plot. Yellow Scale Bar = 2 μm. (B) Same as (A) but for the MT-RNR1 transcript in various transdifferentiated neuron lines (Tdiff.1/2/4). (C) Airyscan immunofluorescence images of the indicated mitochondrial proteins in Tdiff.1 neurons. Yellow Scale Bar = 2 μm. (D) Visualization of representative mitochondrial sequencing reads detected by PNPT1 eCLIP (n=2 replicates) in the indicated neuronal lines. The shaded region denotes the reverse-transcribed MT-ND6 gene. (E) Same as (D) but for SUV3 eCLIP.

Journal: bioRxiv

Article Title: RNA triggers chronic stress during neuronal aging

doi: 10.1101/2025.08.04.668575

Figure Lengend Snippet: (A) Additional airyscan immunofluorescence-FISH images of the mitochondrial marker TOM70 (magenta) and MT-ND6 transcript (green) in Tdiff.1 neurons. Green arrowheads denote cytoplasmic MT-ND6 puncta; the white arrows denote the intensity profiles in the adjacent plot. Yellow Scale Bar = 2 μm. (B) Same as (A) but for the MT-RNR1 transcript in various transdifferentiated neuron lines (Tdiff.1/2/4). (C) Airyscan immunofluorescence images of the indicated mitochondrial proteins in Tdiff.1 neurons. Yellow Scale Bar = 2 μm. (D) Visualization of representative mitochondrial sequencing reads detected by PNPT1 eCLIP (n=2 replicates) in the indicated neuronal lines. The shaded region denotes the reverse-transcribed MT-ND6 gene. (E) Same as (D) but for SUV3 eCLIP.

Article Snippet: The following antibodies were used for eCLIP: 1 μg Rabbit α-PKR (Cell Signaling Technology #12297S); 10 μg Rabbit α-PNPT1 (Bethyl #A303-917A); and 2 μg Rabbit α-SUV3 (Bethyl #A303-056A).

Techniques: Immunofluorescence, Marker, Sequencing, Reverse Transcription